Evaluating the Developed Model of Experimental Rhinitis in Laboratory Rats: Pre-Clinical Experimental Randomized Study

Background. Among the damaging factors affecting mucociliary system of nasal cavity, surgical wound is particular relevance in practice an otorhinolaryngologist. The clinical assessment regeneration associated with certain diffi culties, since intravital morphological examination mucosa patients traumatic. Therefore, development animal models experimental rhinitis considered to be highly relevant order study dynamics pathomorphological changes and assess epithelium regeneration. Objectives. To evaluate developed model laboratory rats by studying clinical, biochemical infl ammatory process. Methods. was tested on 60 mature male Wistar rats. All animals were randomized into two groups: group #1 (n = 30) — which modeling performed #2 control, intact animals. In course experiment, authors examined content CRP blood, evaluated differential blood cell count, studied a morphology septum 2, 5, 10 days after injury process both groups. Statistical analysis results carried out means Statistica 8.0 (StatSoft Inc., USA). Results. After injury, from acute rhinitis, clinically manifested release mucous or mucopurulent secretion nostrils, sneezing scratching nose. An increase CRP, band segmented neutrophils, decrease lymphocytes observed comparison control group. Microscopic showed that phase exudative ammation second day: vascular congestion, edema, neutrophilic infi ltration submucosal membrane against background foci epithelial necrosis. proportion macrophages ltrate increased fi fth day, initial signs restoration tissue formation undifferentiated regenerating appeared tenth day. Conclusion. show adequate have been obtained. characterized manifestations parameters. Particular destructive reparative-proliferative develop as result wound.